Composite

Part:BBa_K2542013

Designed by: Yaoqiang Zheng   Group: iGEM18_NEFU_China   (2018-10-05)


pFig2c-hBax-tCYC1

This plasmid allows a-type yeast to sense the pheromone α factor secreted by α-type yeast. The a-type Saccharomyces cerevisiae expresses Bax protein, which arrests its own cell cycle and reduces cell density.Therefore, the yeast with this plasmid undergoes a decrease in cell density by induction of α factor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 431
    Illegal BamHI site found at 1210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 125
    Illegal BsaI.rc site found at 564


[edit]
Categories
Parameters
None